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By Barbara H. Stuart
Presents an creation to these wanting to exploit infrared spectroscopy for the 1st time, explaining the basic features of this method, easy methods to receive a spectrum and the way to examine infrared info masking a variety of functions. * comprises instrumental and sampling strategies * Covers organic and business functions * comprises compatible questions and difficulties in every one bankruptcy to aid within the research and interpretation of consultant infrared spectra a part of the ANTS (Analytical innovations within the Sciences) sequence.
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Extra info for Infrared Spectroscopy: Fundamentals and Applications
Example text
The spectrum can be regarded as a ‘fingerprint’ of the molecule and so this region is referred to as the fingerprint region. 2 Near-Infrared Region The absorptions observed in the near-infrared region (13 000–4000 cm−1 ) are overtones or combinations of the fundamental stretching bands which occur in the 3000–1700 cm−1 region [1]. The bands involved are usually due to C–H, N–H or O–H stretching. The resulting bands in the near infrared are usually weak in intensity and the intensity generally decreases by a factor of 10 from one overtone to the next.
13, calculate the pathlength of the cell. 5 Reflectance Methods Reflectance techniques may be used for samples that are difficult to analyse by the conventional transmittance methods. Reflectance methods can be divided into two categories. Internal reflectance measurements can be made by using an attenuated total reflectance cell in contact with the sample. There is also a variety of external reflectance measurements which involve an infrared beam reflected directly from the sample surface. 15). A beam of radiation entering a crystal will undergo total internal reflection when the angle of incidence at the interface between the sample and crystal is greater than the critical angle, where the latter is a function of the refractive indices of the two surfaces.
External reflectance measures the radiation reflected from a surface. The material must, therefore, be reflective or be attached to a reflective backing. A particularly appropriate application for this technique is the study of surfaces. 16). The amount of light reflected depends on the angle of incidence, the refractive index, surface roughness and absorption properties of the sample. For most materials, the reflected energy is only 5–10%, but in regions of strong absorptions, the reflected intensity is greater.