By Shan S. Wong
Since the ebook of the 1st version of Chemistry of Protein Conjugation and Cross-Linking in 1991, new cross-linking reagents, significantly multifunctional cross-linkers, were constructed and synthesized. The final touch of the human genome venture has opened a brand new quarter for learning nucleic acid and protein interactions utilizing nucleic acid cross-linking reagents, and advances have additionally been made within the quarter of biosensors and microarray biochips for the detection and research of genes, proteins, and carbohydrates. additionally, advancements in actual recommendations with unheard of sensitivity and determination have facilitated the research of cross-linked items.
Updated to mirror the advances of the twenty first century, this ebook offers:
- An assessment of the chemical ideas underlying the procedures of cross-linking and conjugation
- A thorough checklist of cross-linking reagents released within the literature because the first version, protecting monofunctional, homobifunctional, heterobifunctional, multifunctional, and zero-length cross-linkers
- Reviews of using those reagents in learning protein tertiary buildings, geometric preparations of subunits inside advanced proteins and nucleic acids, near-neighbor research, protein-to-protein or ligand–receptor interactions, and conformational adjustments of biomolecules
- Discusses the appliance of immunoconjugation for immunoassays, immunotoxins for designated remedy, microarray know-how for research of assorted biomolecules, and strong kingdom chemistry for immobilizations