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Example text
In summarizing guidelines for optimization of operating conditions for high pressure ion exchange liquid chromatography, Horvath and Lipsky (1969b) recommended the determination of the following variables: 51 52 3. EXPERIMENTAL METHODS 1. ) 2. Chemical composition of eluents 3. Concentration of eluents 4. pH of eluents 5. Column temperature 6. Length and diameter of column 7. Flow rate 8. Elution modes I. Standardization of Conditions In order to be able to check the reproducibility of the results and the stability of instrument and column conditions, a standard solution should be analyzed routinely.
Fractions can be collected manually or automatically by drop or time. There are many automatic fraction collectors manufactured and almost any of them can be connected to a high pressure liquid Chromatographie system. If liquid chromatography is to be used in conjunction with isotopic labeling, the most convenient setup is to be able to collect the fractions directly in scintillation vials because it eliminates one step in the transferring process. 50 2. INSTRUMENTATION Integrators can be purchased as part of a liquid Chromatographie system or separately from the instrument company or any one of a number of companies that market integrators for gas and liquid chromatographs as well as for other laboratory instruments.
2-15. Application of Microadsorption detectors. Instrument: Varian LCS 1000. 91 mg triolein; (4) amino acid, 450 /ug proline; (5) nucleotide, 50 μ% guanosine 5' monophosphate. Sensitivity: (1) x 32; (2) x 32; (3) x 32; (4) x 1; (5) x 1. Adsorbant: (1) Amb. CG-400; (2) silicic acid; (3) silicic acid; (4) Sephadex G-10; (5) DE-32. 22 M acetic acid. 2 cmVhr; (5) 45 cmVhr. ) 38 2. INSTRUMENTATION to gel filtration chromatography, but the simplicity of operation, ease of cleaning, and freedom from maintainance and calibration make it advantageous for use in other types of chromatography.