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1986d). Among cholinergic projections, the authors revealed, there are two pathways for cholinergic fibre projections to the hippocampus: the largest inputs to the hippocampus are contained in the dorsal pathways (dorsal fornix, fimbria and supracallosal striae), whereas there seems to be another minor subcortical ventral pathway of cholinergic neurons from the diagonal band nucleus. Transsection of the dorsal pathways resulted in the almost complete disappearance of fine GAL-ir fibres in the dorsal portion of the hippocampal formation but were spared in the more ventral sections.
1986b) did not observe any innervation. On the other hand, Melander et al. (1986b) reported few to moderate numbers of GAL-ir cells in deep layers of the cortex at all levels, whereas we have not seen them at anterior levels. Ch'ng et al. (1985) also have seen moderate numbers of GAL-ir cell bodies in the medial parts of the central amygdaloid nucleus, but we and Melander et al. (1986d) believe these cells are located outside of the nucleus at the terminal area of the stria terminalis just medial to the central and dorsal to the basal amygdaloid nucleus.
Ll antiserum diluted 1:60 000 in RIA buffer. Tubes were vortexed and kept at 4 oc for 24 h. 1 RIA buffer to each Galanin in the CNS 33 tube and incubated at 4 oc for 24 h. tl sheep anti-rabbit serum (35% in RIA buffer) were added. Precipitates were allowed to form at 4 oc for 4 h and centrifuged at 5000 g for 30 min. Supernatants were carefully removed by aspiration and the pellets counted in a gamma counter for 2 min each. 2% specific binding. 8. 06 pg/tube. Characterization by HPLC and RIA Three male Sprague-Dawley rats, weighing 200--250 g, were killed by decapitation.