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By E. Heftmann
Chromatography has emerged because the most vital and flexible analytical approach. The ebook is not just an up-to-date model of Heftmann's classical textual content, however it covers parts of destiny value, akin to microfluidics and computing device assets. less than his skilled assistance, professionals in each one box have contributed their functional adventure to an built-in therapy of recent micro research. partly A the theoretical foundation of person separation tools is defined and the technical points are illustrated. It comprises the speculation of gasoline and liquid chromatography in addition to particular chromatographic suggestions, similar to size-exclusion, planar, ion, and affinity chromatography in addition to quite a few electrokinetic separation suggestions. Microfluidics are coated for the 1st time and priceless resources of analytical tools are indexed and evaluated. 1. each one bankruptcy written through an authority2. Thorough remedy of the theoretical foundation of separation methods3. useful consultant for acting analyses
Read or Download Chromatography, Sixth Edition: Fundamentals and applications of chromatography and related differential migration methods - Part A: Fundamentals and technique PDF
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Additional info for Chromatography, Sixth Edition: Fundamentals and applications of chromatography and related differential migration methods - Part A: Fundamentals and technique
Example text
The more methods that are available, the easier and faster one can purify, say, a protein, which is very important in proteomics studies. Similarly, any new phenomenon observed in one of these separation methods has its analog in the other methods. Ignorance of the analogy between different separation techniques has, unfortunately, delayed the progress of separation science and, thereby, also of life sciences and related areas. For instance, isoelectric focusing and indirect detection in electrophoresis were introduced as early as 1961 and 1967, respectively, but the chromatographic counterparts did not appear until 1977 and 1979.
1 Molecular interactions . . . . . . . . . 2 Thermodynamics . . . . . . . . . . 3 Partition . . . . . . . . . . . . 4 Adsorption . . . . . . . . . . . . 5 Complexation . . . . . . . . . . . 6 Ion exchange, ion exclusion, and ion pairing . . . 7 Hydrodynamic and size exclusion . . . . . . 8 Implications . . . . . . . . . . . 5 Optimization of chromatographic separations . . . . . 1 Performance criteria . . . . .
4 Principles of solute-zone separation . . . . . . . 1 Molecular interactions . . . . . . . . . 2 Thermodynamics . . . . . . . . . . 3 Partition . . . . . . . . . . . . 4 Adsorption . . . . . . . . . . . . 5 Complexation . . . . . . . . . . . 6 Ion exchange, ion exclusion, and ion pairing . . . 7 Hydrodynamic and size exclusion . . . . . . 8 Implications . . . . . . . . . . . 5 Optimization of chromatographic separations .