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No standard is required, since the haemoglobin concentration can be calculated from the absorbance, given that the molecular weight and the millimolar extinction coeficient of haemoglobin are known. However, the wavelength of light produced by the instrument must be veriied and the absorbance scale calibrated. A reference solution of cyanmethaemoglobin can be used for calibration. Hb was once measured, in routine practice, by means of a photometer or colorimeter in which light of approximately 540 nm was produced by use of a yellow‐green ilter such as the Ilford 625.
It should be noted that there are hazards associated with determining the microhaematocrit. Glass capillary tubes may break during insertion of modelling clay, leading to a penetrating injury and blood inoculation to the user. In one case human immunodeiciency virus (HIV) Fig. 2 Measurements of packed cell volume (PCV) by the microhaematocrit technique; paired tests from three patients are shown. was transmitted and the house oficer concerned subsequently developed the acquired immune deiciency syndrome (AIDS) [10,11].
A) (b) Fig. 4 Reticulocytes stained with new methylene blue. (a) A group I reticulocyte with a dense clump of reticulum, several group II reticulocytes with a wreath or network of reticulum and several group III reticulocytes with a disintegrated wreath of reticulum. (b) Group II, III and IV reticulocytes: the group IV reticulocyte has two granules of reticulum. There is also a cell with a single dot of reticulum. By some criteria this would also be classiied as a reticulocyte. Continued p. 27 Performing a blood count 27 Fig.