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By Veronika R. Meyer
The 3rd variation of this renowned problem-solving consultant for this widely-used strategy comprises 11 thoroughly new examples and a number of other up to date ones, including as much as over a hundred power pitfalls and error in HPLC. every one instance is gifted on a double web page with the textual content at the left-hand and a determine at the right-hand aspect, precise to the motto "a photo says greater than one thousand words". furthermore, the writer offers crucial basics in addition to worthwhile options, comparable to apparatus checks or caliber coverage processes.
vital source for all these clients and not using a history in analytical chemistry -- displaying how you can use HPLC successfully and procure right effects.
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Extra info for Pitfalls and Errors of HPLC in Pictures, Third Edition
Example text
This approach leads to a certain simplification of the problem as presented in the drawing. If identical volumes of sample and reference solutions are injected, the calibration of the autosampler is irrelevant. , detected at the wrong wavelength, they will both deviate from their ‘‘true’’ values by the same relative amount. If both peaks are of similar size, their shapes and signal-to-noise ratio will be similar, and the integration parameters will be the same anyway. What is important under these circumstances is the repeatability of all steps, including the prerequisite that the mobile phase must not change its properties from one injection to another.
In this region the resolution is slightly lower but the separation is not influenced by small variations in mobile phase composition. 60). Unfortunately obtaining such a perfect calibration graph is not a matter of course because every method is prone to a number of errors. Different and multiple deviations from the ideal are possible: Not enough data points: two points only do not result in a calibration curve! The minimum is three points, and five points should be the rule. ) High scattering: a repeated determination of the calibration curve from the very beginning will make it clear whether the deviations are of a random or systematic nature.
Freshly prepared one – is shown in the figure. 6 mm × 25 cm Chiralcel OD, 10 µm (cellulose tris (3,5-dimethylphenyl-carbamate)) n-heptane/isopropanol 90 : 10 with ≈ 1000 ppm water (top) or anhydrous (bottom), 1 mL min−1 20 ◦ C UV 254 nm Reference: L. Hintermann: J. Org. Chem. 6 Inadequate Purity of Mobile Phase Water For most HPLC analyses, deionized water is not suitable as a component of the mobile phase because it may contain traces of impurities. A dedicated water purification system does a much better job.