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By Terry Mills III, James Conrad Roberson, Christian C. Matchett, Mathew J. Simon, Mark D. Burns, Robert J. Ollis Jr.
Discovering present, distinctive info at the research of drug-related compounds is tough at top. whereas nearly all people engaged within the learn of those compounds has accrued an enormous number of info over the years, a single-source, accomplished assessment of that facts will be a useful source to have. Instrumental facts for Drug research (IDDA), 3rd variation is that source, featuring the most recent details on those compounds in an intensive, common layout. what is new within the 3rd variation:
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Rec. 84, 275 (1942). 7. M . R. Murray and A. P. Stout, Am. J . Anat. 80, 225 (1947). 8. R. L. Ehrmann and G . O . G e y , / Natl. Cancer Inst. 16, 1375 (1956). 9. M . B. Bornstein, Lab Invest. 7, 134 (1958). 10. M . B. Bornstein and M . R. M u r r a y , / Biophys. Biochem. Cytol. 4, 499 (1958). 11. M . B. Bornstein, in "Neurological and Electroencephalographs Correlative Studies in Infancy" (P. Kellaway and I. ), p. 1. Grune & Stratton, N e w York, 1964. 26 I. Neural and Neuromuscular Systems 12.
M y e l i n a t i o n , o b s e r v a b l e i n t h e l i v i n g state, is a feature o f e a c h o f t h e t h r e e c u l ture s y s t e m s d e s c r i b e d , a l t h o u g h differing i n quantity a n d t i m e o f a p p e a r a n c e . M o u s e - d e r i v e d c e r e b e l l a r a n d spinal c o r d e x p l a n t s m y e l i n a t e abundantly, t h e earliest m y e l i n a p p e a r i n g in c e r e b e l l a r cultures, w h e r e a s m u c h less m y e l i n Figure 3 Spinal cord—dorsal root ganglia cultures.
I n this r e a g g r e g a t e system, e m b r y o n i c brain r e g i o n s are d i s s o c i a t e d i n t o single c e l l suspensions. T h e c e l l s are p l a c e d i n c u l t u r e m e d i u m i n flasks w h i c h are rotated i n an i n c u b a t o r . O v e r t h e n e x t 24 hr, t h e e m b r y o n i c c e l l s a d h e r e t o o n e a n o t h e r t o f o r m reaggregates: small, r o u g h l y spherical clusters o f c e l l s w h i c h are 3 0 0 t o 4 0 0 jitm i n d i a m e t e r (Fig.