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By Eberhard Neumann, Sergej Kakorin, Katja Toensing (auth.), Mark J. Jaroszeski PhD, Richard Heller PhD, Richard Gilbert PhD (eds.)
In Electrochemotherapy, Electrogenetherapy, and Transdermal Drug supply, major specialists comprehensively overview all elements of the supply of healing molecules to cells utilizing electric impulses-i.e., the terribly promising new components of electrogene treatment, electrochemotherapy, and transdermal drug supply. Their survey levels from outlines of the elemental actual ideas that govern mobile permeabilization via pulsed electrical fields, to descriptions of the present cutting-edge in instrumentation and electrodes, to summaries of preclinical and scientific trial effects. The authors concentrate on drug, gene, and transdermal supply concepts, supplying targeted examples of drug supply utilizing electrical fields from a number of pulse turbines and electrodes. The protocols hide virtually each version in process and/or gear in order that investigators can quite simply determine the strategy wanted for his or her personal paintings, or adapt one to their specific purposes.
finished and authoritative, Electrochemotherapy, Electrogenetherapy, and Transdermal Drug supply presents entré to an immensely useful set of in vivo electroporation ideas, together with electrogene treatment, electrochemotherapy, and transdermal drug delivery-techniques retaining nice promise for all these operating towards higher remedies for in melanoma, metabolic ailments, and vaccination.
Read or Download Electrochemotherapy, Electrogenetherapy, and Transdermal Drug Delivery: Electrically Mediated Delivery of Molecules to Cells PDF
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Extra resources for Electrochemotherapy, Electrogenetherapy, and Transdermal Drug Delivery: Electrically Mediated Delivery of Molecules to Cells
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1986) Chemical electric field effects in biological macromolecules. Prog. Biophys. Mol. Biol. 47, 197–231. Steiner, U. and Adam, G. (1984) Interfacial properties of hydrophilic surfaces of phospholipid films as determined by method of contact angles. Cell Biophys. 6, 279–299. , and Huber, R. (1997) Annexin V and vesicle membrane electroporation. Eur. Biophys. J. 26, 307–318. Seifert, U. and Lipowsky, R. (1995) Morphology of vesicles. Structure and Dynamics of Membranes, Vol. 1A (Lipowsky, R. ), Elsevier, Amsterdam, pp.
Increased spontaneous curvature can either hinder or facilitate ME (30). For instance, the Ca2+ mediated adsorption of the protein annexinV to anionic lipids increases the lipid packing density by insertion of the tryptophan side chain into the membrane surface. This in turn, reduces the electroporatability of the remaining membrane parts (30). Alternatively, the adsorption of plasmid DNA on the membrane surface, mediated by calcium or sphingosine, obviously facilitates ME and thus the transport of small ions (leak) and DNA itself across the membrane (10,37,38).
Pharmacol. 27, 745–748. , and Mir, L. (1998) Enhancement of cytoxicity by electropermeabilization: An improved method for screening drugs. Anticancer Drugs 9, 319–325. Principles of Electroporation and Transport 35 44. , and Reintgen, D. (1996) Phase I/II trial for the treatment of cutaneous and subcutaneous tumors using electrochemotherapy. Cancer 77, 964–971. Instrumentation and Electrodes 37 2 Instrumentation and Electrodes for In Vivo Electroporation Gunter A. Hofmann 1. Introduction Electroporation (EP) of drugs and genes into cells in vitro became a standard procedure in molecular biology laboratories in the last decade.