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By Alton Meister
Advances in Enzymology and similar components of Molecular Biology is a seminal sequence within the box of biochemistry, delivering researchers entry to authoritative reports of the newest discoveries in all parts of enzymology and molecular biology. those landmark volumes date again to 1941, supplying an unmatched view of the ancient improvement of enzymology. The sequence bargains researchers the most recent realizing of enzymes, their mechanisms, reactions and evolution, roles in advanced organic method, and their program in either the laboratory and undefined. every one quantity within the sequence positive aspects contributions by means of best pioneers and investigators within the box from world wide. All articles are conscientiously edited to make sure thoroughness, caliber, and clarity.
With its wide selection of issues and lengthy historic pedigree, Advances in Enzymology and similar parts of Molecular Biology can be utilized not just by means of scholars and researchers in molecular biology, biochemistry, and enzymology, but additionally by means of any scientist drawn to the invention of an enzyme, its houses, and its functions.
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Temple, Nature, 163, 859 (1949). 48. Huggins, M. ,Chem. , 32, 195 (1943). 49. ,and J. A m . Chem. , 72,2899(1950). 50. , and A. Street, Phil. Trans. Roy. 230, 75 (1931). 51. , T. Shimanouchi, M. Tsuboi, T. Sugita, E. Kato, and E. Kondo, J . Am. Chem. ,73, 1330 (1951). 52. ,T. Shimanouchi, M. Tsuboi, T. Sugita, K. Kurosaki, N. Soda, J. Am. Chem. , 74, 4639 (1952). 53. , T. Shimanouchi, M. Tsuboi, K. Kuratani, T. Sugita, N. Soda, J. Am. Chem. , 76, 1863 (1953). 54. Tsuboi, T. Shimanouchi, T. Sugita, and T.
Japan, 27, 287 (1954). 62. ,J. Am. Chem. ,72, 1499 (1950). 63. ,T. Shimanouchi, and S. Mirushima, J. Chem. , 26, 608 (1957). 64. , to be published. 65. , T. Shimanouchi, and S. Mizushima, Bull. Chem. Japan, 29, 802 (1956). 66. , T. Shimanouchi, K. Kuratani, M. Tsuboi, T. Sugita, I. Nakagawa, and K. Kurosaki, Nature, 169, 1058 (1952). Advances in Enzymology and Related Areas of Molecular Biology, Volume 23 Edited by F. F. Nord Copyright © 1961 by Interscience Publishers, Inc. DENATURATION AND INACTIVATION O F ENZYME P R O T E I N S By KAZUO OKUNUKI, Osaka, Japan CONTENTS I.
C. Effect of TCA Treatment and Boiling on the Autoxidizability of Cytochrome c . ......................................... 29 30 32 32 32 35 35 37 37 38 39 39 41 43 44 44 44 46 48 50 58 62 62 64 30 KAXUO OKUNUKI CONTENTS (continued) D. Effect of Digestion by Bacterial Proteinase on the Absorption Spectra of Yeast Cytochrome c . E. Mode of Existence of Native and ModSed Cytochrome c . , . . F. Ultraviolet Absorption Spectrum of Crystalline Bovine Cytochrome c in its Oxidized and Reduced Forms..