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Additional resources for Protein Synthesis - Methods and Protocols
Example text
For example, iron can be displaced from ion-exchange columns by passage of sodium citrate or sodium ethylenediaminetetraacetate. Some of the more common commercially available resins are listed in Table 13. Ion-exchange resins swell in water to an extent which depends on the amount of crosslinking in the polymer, so that columns should be prepared from the wet material by adding it as a suspension in water to a tube already partially filled with water. /ml of wet resin. 6-2 for most strong-acid or strong-base types.
In this way a very large number of liquid-liquid extractions can be carried out automatically. The closer the ionisation constants of the impurities are to those of the required material, the larger should the be the number of extractions to effect a good separation. Casinovi's review, "A Comprehensive Bibliography of Separations of Organic Substances by Countercurrent Distribution" in Chromatographic Reviews 5 161 1963, and references on p. 47 under Solvents, Solvent Extraction and Distribution.
The affinity method may be biospecific, for example as an antibody-antigen interaction chemical as in the chelation of boronate by cis-diols, or of unknown origin as in the binding of certain dyes to albumin. Hydrophobic adsorption chromatography takes advantage of the hydrophobic properties of substances to be separated and has also found use in biochemistry (Hoftsee BBRC 50 75 1 1973 ; Jennissen and Heilmayer Jr Biochemistry 14 754 1975). Cabra1, lmmobilised Enzymes and Cells: A Laboratory Manual, Adam Hilger, Bristol, 1987).