PCR Methods in Foods by Amanda Fairchild M.S., Margie D. Lee DVM, Ph.D. (auth.), Dr.

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By Amanda Fairchild M.S., Margie D. Lee DVM, Ph.D. (auth.), Dr. John Maurer (eds.)

This e-book is a primer for college students, school, and different execs attracted to molecular biology and its integration into nutrition protection, through introducing the reader to diagnostic PCR-based applied sciences utilized in detection of pathogens in meals.

This booklet will let its reader to:

  1. Understand the foundations in the back of PCR together with real-time
  2. Know the fundamentals thinking about the layout, optimization, and implementation of PCR in foodstuff microbiology lab setting
  3. Interpret results
  4. Know barriers and strengths of PCR
  5. Understand the fundamental ideas at the back of microarrays and its power functions in nutrients microbiology.

Figures, charts, and tables support illustrate techniques and supply the reader with a major start line in bringing molecular diagnostics into the meals microbiology lab.

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E. A. D. Andreadis. 2004. Nucleic acid amplification strategies for DNA microarray-based pathogen detection. 70:3047–3054. , W. R. Reeves. 2001. Sequence analysis of four Shigella boydii O-antigen loci: Implication for Escherichia coli and Shigella relationships. Infect. Immun. 69:6923–6930. R. Reeves. 1998. Organization of Escherichia coli O157 O antigen gene cluster and identification of its specific genes. Infect. Immun. 66:3545–3551. H. Crick. 1953. Molecular structure of nucleic acids; a structure for deoxyribose nucleic acid.

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Extended survival and persistence of Campylobacter spp. in water and aquatic biofilms and their detection by immunofluorescent-antibody and rRNA staining. Appl. Environ. Microbiol. 64:733–741. 12. H. A. F. Smyser. 1967. A comparison of incubation time and temperature for the isolation of Salmonella. Avian Dis. 11:217–225. REFERENCES 37 13. W. N. Cutter. 2003. Comparison of recovery methods for freeze-injured Listeria monocytogenes, Salmonella Typhimurium, and Campylobacter coli in cell suspensions and associated with pork surfaces.

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